Feature Publications

 
Featured
Nutrient stress is a target for new antibiotics

Authors: Carfrae LA, Brown ED

Reference: Trends Microbiol. 2023 January 27; doi: 10.1016/j.tim.2023.01.002

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Genetic and Chemical Screening Reveals Targets and Compounds to Potentiate Gram-Positive Antibiotics against Gram-Negative Bacteria

Authors: Klobucar K, Jardine E, Farha MA, MacKinnon MR, Fragis M, Nkonge B, Bhando T, Borrillo L, Tsai CN, Johnson JW, Coombes BK, Magolan J, Brown ED.

Reference: ACS Infect. Dis. 2022 September 13; doi: 10.1021/acsinfecdis.2c00357

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Synthetic Genetic Interactions Reveal a Dense and Cryptic Regulatory Network of Small Noncoding RNAs in Escherichia coli

Authors: Rachwalski K, Ellis MJ, Tong M, Brown ED.

Reference: MBio 2022 August 3; doi: 10.1128/mbio.01225-22

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Preclinical Development of Pentamidine Analogs Identifies a Potent and Nontoxic Antibiotic Adjuvant

Authors: MacNair CR, Farha MA, Serrano-Wu MH, Lee KK, Hubbard B, Côté JP, Carfrae LA, Tu MM, Gaulin JL, Hunt DK, Hung DT, Brown ED.

Reference: ACS Infect. Dis. 2022 March 23; doi: 10.1021/acsinfecdis.1c00482

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Chemical Screen for Vancomycin Antagonism Uncovers Probes of the Gram-Negative Outer Membrane

Authors: Klobucar K, Côté JP, French S, Borrillo L, Guo ABY, Serrano-Wu MH, Lee KK, Hubbard B, Johnson JW, Gaulin JL, Magolan J, Hung DT, and Brown ED.

Reference: ACS Chem. Biol. 2021 May 11; doi: 10.1021/acschembio.1c00179

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Systems-Level Chemical Biology to Accelerate Antibiotic Drug Discovery

Authors: Farha MA, French S, and Brown ED.

Reference: Acc. Chem. Res.. 2021 Mar 31; doi: 10.1021/acs.accounts.1c00011

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2023

Featured
Nutrient stress is a target for new antibiotics

Authors: Carfrae LA, Brown ED

Reference: Trends Microbiol. 2023 January 27; doi: 10.1016/j.tim.2023.01.002

CARD 2023: expanded curation, support for machine learning, and resistome prediction at the Comprehensive Antibiotic Resistance Database

Authors: Alcock BP, Huynh W … Bhando B, Carfrae LA, Farha MA, French S, Gordzevich R, Rachwalski K, Tu MM … Brown ED … McArthur AG.

Reference: Nucleic Acids Res. 2023 January 6; doi: 10.1093/nar/gkac920

An Omniphobic Spray Coating Created from Hierarchical Structures Prevents the Contamination of High-Touch Surfaces with Pathogens

Authors: Abu Jarad N, Rachwalski K, Bayat F, Khan S, Shakeri A, MacLachlan R, Villegas M, Brown ED, Soleymani L, Didar TF

Reference: Small 2023 January 1; doi: 10.1002/smll.202205761

2022

Featured
Central metabolism is a key player in E. coli biofilm stimulation by sub-MIC antibiotics

Authors: Yaeger LN, French S, Brown ED, Côté JP, Burrows LL

Reference: bioRxiv 2022 September 15; doi: 10.1101/2022.09.14.507886

Genetic and Chemical Screening Reveals Targets and Compounds to Potentiate Gram-Positive Antibiotics against Gram-Negative Bacteria

Authors: Klobucar K, Jardine E, Farha MA, MacKinnon MR, Fragis M, Nkonge B, Bhando T, Borrillo L, Tsai CN, Johnson JW, Coombes BK, Magolan J, Brown ED.

Reference: ACS Infect. Dis. 2022 September 13; doi: 10.1021/acsinfecdis.2c00357

A genetic platform to investigate the functions of bacterial drug efflux pumps

Authors:Teelucksingh T, Thompson LK, Zhu S, Kuehfuss NM., Goetz JA, Gilbert SE, MacNair CR, Geddes-McAlister J, Brown ED, Cox G.

Reference: Nat. Chem. Biol. 2022 September 5; doi: 10.1038/s41589-022-01119-y

Polynuclear ruthenium complexes are effective antibiotics against Pseudomonas aeruginosa

Authors: Weber BS, Carfrae LA, Woods JJ, Klobucar K, Bigham NP., MacNair CR, Raivio TL, Wilson JJ, Brown ED.

Reference: bioRxiv 2022 August 14; doi: 10.1101/2022.08.11.503708

Synthetic Genetic Interactions Reveal a Dense and Cryptic Regulatory Network of Small Noncoding RNAs in Escherichia coli

Authors: Rachwalski K, Ellis MJ, Tong M, Brown ED.

Reference: MBio 2022 August 3; doi: 10.1128/mbio.01225-22

Preclinical Development of Pentamidine Analogs Identifies a Potent and Nontoxic Antibiotic Adjuvant

Authors: MacNair CR, Farha MA, Serrano-Wu MH, Lee KK, Hubbard B, Côté JP, Carfrae LA, Tu MM, Gaulin JL, Hunt DK, Hung DT, Brown ED.

Reference: ACS Infect. Dis. 2022 March 23; doi: 10.1021/acsinfecdis.1c00482

Transparent and Highly Flexible Hierarchically Structured Polydimethylsiloxane Surfaces Suppress Bacterial Attachment and Thrombosis Under Static and Dynamic Conditions

Authors: Khan S, Abu Jarad N, Ladouceur L, Rachwalski K, Bot V, Shakeri A, Maclachlan R, Sakib S, Weitz JI, Brown ED, Soleymani L, Didar TF.

Reference: Small 2022 February 28; doi: 10.1002/smll.202108112

New potentiators of ineffective antibiotics: Targeting the Gram-negative outer membrane to overcome intrinsic resistance

Authors: Klobucar K, Brown ED.

Reference: Curr. Opin. Chem. Biol. 2022 February 1; doi: 10.1016/j.cbpa.2021.102099

Antibacterial Activity of Metergoline Analogues: Revisiting the Ergot Alkaloid Scaffold for Antibiotic Discovery

Authors: Johnson JW, Ellis MJ, Piquette ZA, MacNair C, Carfrae L, Bhando T, Ritchie NE, Saliba P, Brown ED, Magolan J.

Reference: ACS Med. Chem. Lett. 2022 January 21; doi: 10.1021/acsmedchemlett.1c00648

2021

Featured
Structural Insights into the Inhibition of Undecaprenyl Pyrophosphate Synthase from Gram-Positive Bacteria

Authors: Workman SD, Day J, Farha MA, El Zahed SS, Bon C, Brown ED, Organ MG, Strynadka, NC.

Reference: J. Med. Chem. 2021 September 2; doi: 10.1021/acs.jmedchem.1c00941

Armeniaspirols inhibit the AAA+ proteases ClpXP and ClpYQ leading to cell division arrest in Gram-positive bacteria

Authors: Labana P, Dornan MH, Lafrenière, Czarny TL, Brown ED, Pexacki JP, Boddy CN.

Reference: Cell Chem. Biol. 2021 July 21; doi: 10.1016/j.chembiol.2021.07.001

A Staphylococcus aureus clpX Mutant Used as a Unique Screening Tool to Identify Cell Wall Synthesis Inhibitors that Reverse Beta-Lactam Resistance in MRSA

Authors: Bæk KT, Jensen C, Farha MA, Nielsen TK, Paknejadi E, Mebus VH, Vestergaard M, Brown ED, Frees D

Reference: Front. Mol. Biosci. 2021 June 4; doi: 10.3389/fmolb.2021.691569

Chemical Screen for Vancomycin Antagonism Uncovers Probes of the Gram-Negative Outer Membrane

Authors: Klobucar K, Côté JP, French S, Borrillo L, Guo ABY, Serrano-Wu MH, Lee KK, Hubbard B, Johnson JW, Gaulin JL, Magolan J, Hung DT, and Brown ED.

Reference: ACS Chem. Biol. 2021 May 11; doi: 10.1021/acschembio.1c00179

Crystallographic analysis of TarI and TarJ, a cytidylyltransferase and reductase pair for CDP-ribitol synthesis in Staphylococcus aureus wall teichoic acid biogenesis

Authors: Li KKF, Gale RT, Petrochenko EV, Borchers CH, Brown ED, Strynadka NCJ.

Reference: JSB 2021 April 2; doi: 10.1016/j.jsb.2021.107733

Systems-Level Chemical Biology to Accelerate Antibiotic Drug Discovery

Authors: Farha MA, French S, and Brown ED.

Reference: Acc. Chem. Res.. 2021 Mar 31; doi: 10.1021/acs.accounts.1c00011

Physicochemical and Structural Parameters Contributing to the Antibacterial Activity and Efflux Susceptibility of Small-Molecule Inhibitors of Escherichia coli

Authors: El Zahed SS, French S, Farha MA, Kumar G, and Brown ED.

Reference: AAC. 2021 Jan 19; doi: 10.1128/AAC.01925-20.

2020

Featured
Phage-antibiotic combinations: a promising approach to constrain resistance evolution in bacteria

Authors: North OI and Brown ED

Reference: Ann N Y Acad Sci. 2020 Nov 11; doi: 10.1111/nyas.14533

Gene Dispensability in Escherichia coli Grown in Thirty Different Carbon Environments

Authors: Tong M, French S, El Zahed SS, Ong WK, Karp PD, Brown ED

Reference: mBio. 2020 Sept 29; doi: 10.1128/mBio.02259-20

Outer Membrane Disruption Overcomes Intrinsic, Acquired, and Spontaneous Antibiotic Resistance

Authors: MacNair CR, Brown ED

Reference: mBio. 2020 Sept 22; doi: 10.1128/mBio.01615-20

Overcoming Acquired and Native Macrolide Resistance with Bicarbonate

Authors: Farha MA, MacNair CR, Carfrae LA, El Zahed SS, Ellis MJ, Tran HKR, McArthur AG, Brown ED

Reference: ACS Infect. Dis. 2020 Sept 08; doi: 10.1021/acsinfecdis.0c00340

Genetic and Chemical Screening in Human Blood Serum Reveals Unique Antibacterial Targets and Compounds against Klebsiella pneumoniae

Authors: Weber BS, De Jong AM, Guo ABY, Dharavath S, French S, Fiebig-Comyn AA, Coombes BK, Magnolan J, Brown ED.

Reference: Cell Rep. 2020 July 21; doi: 10.1016/j.celrep.2020.107927

Genetic and Chemical-Genetic Interactions Map Biogenesis and Permeability Determinants of the Outer Membrane of Escherichia coli

Authors: Klobucar K, French S, Côté JP, Howes JR, Brown ED

Reference: mBio. 2020 Mar 10; doi: 10.1128/mBio.00161-20

Uncovering the Hidden Antibiotic Potential of Cannabis

Authors: Farha MA*, El-Halfawy OM*, Gale RT, MacNair CR, Carfrae LA, Zhang X, Jentsch NG, Magolan J, Brown ED.

Reference: ACS Infect. Dis. 2020 Feb 04; doi: 10.1021/acsinfecdis.9b00419

A comprehensive guide to dynamic analysis of microbial gene expression using the 3D-printed PFIbox and a fluorescent reporter library

Authors: French S, Guo ABY, Brown ED.

Reference: Nat. Protoc. 2020 Jan 08; doi: 10.1038/s41596-019-0257-0

2019

Featured
Flexible Hierarchical Wraps Repel Drug-Resistant Gram-Negative and Positive Bacteria

Authors: Imani SM, Maclachlan R, Rachwalski K, Chan Y, Lee L, McInnes M, Grandfield K, Brown ED, Didar TF, Soleymani L

Reference: ACS Nano. 2019 December 13; doi: 10.1021/acsnano.9b06287

Discovery of an antivirulence compound that reverses β-lactam resistance in MRSA

Authors: El-Halfawy OM, Czarny TL, Flannagan RS, Day J, Bozelli JC, Kuiack RC, Salim A, Eckert P, Epand RM, McGavin MJ, Organ MG, Heinrichs DE, Brown ED.

Reference: Nat. Chem. Biol. 2019 Nov 25; doi: 10.1038/s41589-019-0401-8

Creative targeting of the Gram‐negative outer membrane in antibiotic discovery

Authors: MacNair CR, Tsai CN, Brown ED.

Reference: Ann. N. Y. Acad. Sci. 2019 Nov 24; doi: 10.1111/nyas.14280

Mimicking the human environment in mice reveals that inhibiting biotin biosynthesis is effective against antibiotic-resistant pathogens

Authors: Carfrae LA, MacNair CR, Brown CM, Tsai CN, Weber BS, Zlitni S, Rao VN, Chun J, Junop MS, Coombes BK, Brown ED.

Reference: Nat. Microbiol. 2019 Oct 28; doi: 10.1038/s41564-019-0595-2

Potentiation of Antibiotics against Gram-Negative Bacteria by Polymyxin B Analogue SPR741 from Unique Perturbation of the Outer Membrane

Authors: French S, Farha M, Ellis MJ, Sameer Z, Côté JP, Cotroneo N, Lister T, Rubio A, Brown ED.
Reference: ACS Infect. Dis. 2019 Sept 30th; doi: 10.1021/acsinfecdis.9b00159

Drug repurposing for antimicrobial discovery.

Authors: Farha MA, Brown ED.
Reference: Nat. Microbiol. 2019 Mar 04; doi: 10.1038/s41564-019-0357-1

A macrophage-based screen identifies antibacterial compounds selective for intracellular Salmonella Typhimurium

Authors: Ellis MJ, Tsai CN, Johnson JW, French S, Elhenawy W, Porwollik S, Andrews-Polymenis H, McClelland M, Magolan J, Coombes BK and Brown ED. 
Reference: Nat. Commun. 2019 Jan 14; doi: 10.1038/s41467-018-08190-x

2018

Featured
Open-Source High-Throughput Phenomics of Bacterial Promoter-Reporter Strains

Authors: French S, Coutts BE, Brown ED.
Reference: Cell Syst. 2018. Aug 29; doi:10.1016/j.cels.2018.07.004

Nutrient Stress Small-Molecule Screening Platform for Escherichia coli

Authors: El Zahed SS, Kumar G, Tong M, Brown ED.
Reference: Bridget Wagner (ed.), Phenotypic Screening: Methods and Protocols, Methods in Molecular Biology, vol. 1787, doi:10.1007/978-1-4939-7847-2_1

Chemical-Chemical Combinations Map Uncharted Interactions in Escherichia coli under Nutrient Stress

Authors: El Zahed SS, Brown ED.
Reference: iScience. 2018 Mar 27; doi: 10.1016/j.isci.2018.03.018.

Silent but deadly: IS200 promotes pathogenicity in Salmonella Typhimurium

Authors: Ellis MJ, Carfrae LA, MacNair CR, Trussler RS, Brown ED, Haniford DB.
Reference: RNA Biol. 2018 Feb 1;15(2):176-181. doi: 10.1080/15476286.2017.1403001.

Overcoming mcr-1 mediated colistin resistance with colistin in combination with other antibiotics

Authors: MacNair CR, Stokes JM, Carfrae LA, Fiebig-Comyn AA, Coombes BK, Mulvey MR, Brown ED.
Reference: Nature Comm. 2018 Jan 31;9(1):458. doi:10.1038/s41467-018-02875-z

2017

Featured
B. subtilis LytR-CpsA-Psr Enzymes Transfer Wall Teichoic Acids from Authentic Lipid-Linked Substrates to Mature Peptidoglycan In Vitro

Authors: Gale RT, Li KKL, Sun T, Strynadka NCJ, Brown ED.
Reference: Cell Chem Biol. 2017 Dec 1;24(12):1537-1546. doi: 10.1016/j.chembiol.2017.09.006.

Bicarbonate alters bacterial susceptibility to antibiotics by targeting the proton motive force

Authors: Farha MA, French S, Stokes JM, Brown ED.
Reference: ACS Infect Dis. 2017 Dec 21;4(3):382-390. doi: 10.1021/acsinfecdis.7b00194.

Exploiting the sensitivity of nutrient transporter deletion strains in discovery of natural product antimetabolites

Authors: Gehrke SS, Kumar G, Yokubynas NA, Côté JP, Wang W, French S, MacNair CR, Wright GD,
Reference: ACS Infect Dis. 2017 Oct 25;3(12):955-965. doi: 10.1021/acsinfecdis.7b00149.

Use of genetic and chemical synthetic lethality as probes of complexity in bacterial cell systems

Authors: Klobucar K, Brown ED.
Reference: FEMS Microbiol Rev. 2017 Oct 24;42(1):fux054. doi: 10.1093/femsre/fux054.

Chemical genomics reveals mechanistic hypotheses for uncharacterized bioactive molecules in bacteria

Authors: French S, Ellis MJ, Coutts BE, Brown ED.
Reference: Curr Opin Microbiol. 2017 Sept 26;39:42-47. doi: 10.1016/j.mib.2017.09.005

Bacteria Getting into Shape: Genetic Determinants of E. coli Morphology

Authors: French S, Côté JP, Stokes JM, Truant R, Brown ED.
Reference: mBio. 2017 Mar 7;8(2):e01977-16. doi: 10.1128/mBio.01977-16

Pentamidine sensitizes Gram-negative pathogens to antibiotics and overcomes acquired colistin resistance

Authors: Stokes JM, MacNair CR, Ilyas B, French S, Côté JP, Bouwman C, Farha MA, Sieron AO, Whitfield C, Coombes BK, Brown, ED.
Reference: Nature Microbiology. 2017 Mar 06;2(5):17028. doi:10.1038/nmicrobiol.2017.28

2016

Featured
A cell-based approach to characterize antimicrobial compounds through kinetic dose response

Authors: MacNair CR, Stokes JM, French S, Myers CL, Iyer KR, Brown ED.
Reference: Bioorg Med Chem. 2016 Dec 15;24(24):6315-6319. doi: 10.1016/j.bmc.2016.09.053.

Structure and Mechanism of Staphylococcus aureus TarS, the Wall Teichoic Acid β-glycosyltransferase Involved in Methicillin Resistance

Authors: Sobhanifar S, Worrall LJ, King DT, Wasney GA, Baumann L, Gale RT, Nosella M, Brown ED, Withers SG, Strynadka NC.
Reference: PLoS Pathog. 2016 Dec 14;12(12):e1006067. doi: 10.1371/journal.ppat.1006067.

Identification of Two Phosphate Starvation-induced Wall Teichoic Acid Hydrolases Provides First Insights into the Degradative Pathway of a Key Bacterial Cell Wall Component.

Authors: Myers CL, Li FK, Koo BM, El-Halfawy OM, French S, Gross CA, Strynadka NC, Brown ED.
Reference: J Biol Chem. 2016 Dec 9;291(50):26066-26082.

The Genome-Wide Interaction Network of Nutrient Stress Genes in Escherichia coli

Authors: Côté JP, French S, Gehrke SS, MacNair CR, Mangat CS, Bharat A, Brown ED.
Reference: MBio. 2016 Nov 22;7(6). pii: e01714-16. doi: 10.1128/mBio.01714-16.

Systematic Genetic Screens Reveal the Dynamic Global Functional Organization of the Bacterial Translation Machinery

Authros: Gagarinova A, Stewart G, Samanfar B, Phanse S, White CA, Aoki H, Deineko V, Beloglazova N, Yakunin AF, Golshani A, Brown ED, Babu M, Emili A.
Reference: Cell Rep. 2016 Oct 11;17(3):904-916. doi: 10.1016/j.celrep.2016.09.040.

A Comprehensive, CRISPR-based Functional Analysis of Essential Genes in Bacteria

Authors: Peters JM, Colavin A, Shi H, Czarny TL, Larson MH, Wong S, Hawkins JS, Lu CH, Koo BM, Marta E, Shiver AL, Whitehead EH, Weissman JS, Brown ED, Qi LS, Huang KC, Gross CA
Reference: Cell. 2016 Jun 2;165(6):1493-506 

Potentiation of Aminoglycoside Activity in Pseudomonas aeruginosa by Targeting the AmgRS Envelope Stress-Responsive Two-Component System.

Authors: Poole K, Gilmour C, Farha MA, Mullen E, Lau CH, Brown ED.

Reference: Antimicrob Agents Chemother. 2016 May 23;60(6):3509-18. 

A Small-Molecule Screening Platform for the Discovery of Inhibitors of Undecaprenyl Diphosphate Synthase

Authors: Tomasz L. Czarny, Eric D. Brown

Reference: ACS Infect. Dis. May 7 2016, DOI: 10.1021/acsinfecdis.6b00044 

Strategies for target identification of antimicrobial natural products

Authors: Farha MA, Brown ED
Reference: Nat Prod Rep. 2016 May 4;33(5):668-80. doi: 10.1039/c5np00127g.

Structural and Kinetic Characterization of Diazabicyclooctanes as Dual Inhibitors of Both Serine-β-Lactamases and Penicillin-Binding Proteins

Authors:  King AM, King DT, French S, Brouillette E, Asli A, Alexander JA, Vuckovic M, Maiti SN, Parr TR Jr, Brown ED, Malouin F, Strynadka NC, Wright GD.

Reference: ACS Chem Biol. 2016 Apr 15;11(4):864-8. doi: 10.1021/acschembio.5b00944. Epub 2016 Jan 14.

Assembly and clustering of natural antibiotics guides target identification.

Authors:  Johnston CW, Skinnider MA, Dejong CA, Rees PN, Chen GM, Walker CG, French S, Brown ED, Bérdy J, Liu DY, Magarvey NA.

Reference: Nat Chem Biol. 2016 Apr;12(4):233-9. doi: 10.1038/nchembio.2018. Epub 2016 Feb 1.

A robust platform for chemical genomics in bacterial systems

Authors: French S, Mangat C, Bharat A, Côté JP, Mori H, Brown ED.
Reference: Mol Biol Cell. 2016 Mar 15;27(6):1015-25. doi: 10.1091/mbc.E15-08-0573. Epub 2016 Jan 20.

Cold Stress Makes Escherichia coli Susceptible to Glycopeptide Antibiotics by Altering Outer Membrane Integrity.

Authors: Stokes JM, French S, Ovchinnikova OG, Bouwman C, Whitfield C, Brown ED.

Reference: Cell Chem Biol. 2016 Feb 18;23(2):267-77

Antibacterial drug discovery in the resistance era

Authors: Brown ED, Wright GD. 
Reference: Nature. 2016 Jan 21;529(7586):336-43. doi: 10.1038/nature17042.

2015

Featured
Chemical modulators of ribosome biogenesis as biological probes

Authors: Stokes JM, Brown ED. 
Reference: Nat Chem Biol. 2015 Dec;11(12):924-32. doi: 10.1038/nchembio.1957. Epub 2015 Nov 17.

New chemical tools to probe cell wall biosynthesis in bacteria

Authors: Gale RT, Brown ED
References: Curr Opin Microbiol. 2015 Oct;27:69-77. doi: 10.1016/j.mib.2015.07.013. Epub 2015 Aug 25.

Editorial overview: Microbial systems biology.

Authors:  Brown ED, Typas A.
Reference: Curr Opin Microbiol. 2015 Oct;27:viii-ix. doi: 10.1016/j.mib.2015.09.007. Epub 2015 Oct 20.

Unconventional screening approaches for antibiotic discovery.

Authors: Farha MA, Brown ED. 
Reference: Ann N Y Acad Sci. 2015 Sep;1354:54-66. doi: 10.1111/nyas.12803. Epub 2015 Jun 22.

Antagonism screen for inhibitors of bacterial cell wall biogenesis uncovers an inhibitor of undecaprenyl diphosphate synthase

Authors: Farha MA, Czarny TL, Myers CL, Worrall LJ, French S, Conrady DG, Wang Y, Oldfield E, Strynadka NC, Brown ED.
Reference: Proc Natl Acad Sci U S A. 2015 Sep 1;112(35):11048-53. doi: 10.1073/pnas.1511751112. Epub 2015 Aug 17.

An electron transfer flavoprotein is essential for viability and its depletion causes a rod-to-sphere change in Burkholderia cenocepacia.

Authors: Bloodworth RA, Zlitni S, Brown ED, Cardona ST.
Reference: Microbiology. 2015 Oct;161(10):1909-20. doi: 10.1099/mic.0.000156. Epub 2015 Aug 6.

Characterization of Wall Teichoic Acid Degradation by the Bacteriophage ϕ29 Appendage Protein GP12 Using Synthetic Substrate Analogs

Authors: Myers CL, Ireland RG, Garrett TA, Brown ED.
Reference: J Biol Chem. 2015 Jul 31;290(31):19133-45. doi: 10.1074/jbc.M115.662866. Epub 2015 Jun 17.

A Pipeline for Screening Small Molecules with Growth Inhibitory Activity against Burkholderia cenocepacia

Authors: Selin C, Stietz MS, Blanchard JE, Gehrke SS, Bernard S, Hall DG, Brown ED, Cardona ST.
Reference: PLoS One. 2015 Jun 8;10(6):e0128587. doi: 10.1371/journal.pone.0128587. eCollection 2015.

Chemical inhibition of bacterial ribosome biogenesis shows efficacy in a worm infection model

Authors: Stokes JM, Selin C, Cardona ST, Brown ED.
Reference: Antimicrob Agents Chemother. 2015 May;59(5):2918-20. doi: 10.1128/AAC.04690-14. Epub 2015 Feb 23.

Structure and mechanism of Staphylococcus aureus TarM, the wall teichoic acid α-glycosyltransferase.

Authors: Sobhanifar S, Worrall LJ, Gruninger RJ, Wasney GA, Blaukopf M, Baumann L, Lameignere E, Solomonson M, Brown ED, Withers SG, Strynadka NC.
Reference: Proc Natl Acad Sci U S A. 2015 Feb 10;112(6):E576-85. doi: 10.1073/pnas.1418084112. Epub 2015 Jan 26.

2014

Featured
A dual reporter system for detecting RNA interactions in bacterial cells.

Authors: Kuryllo K, Jahanshahi S, Zhu W, Brown ED, Li Y.
Reference: Chembiochem. 2014 Dec 15;15(18):2703-9. doi: 10.1002/cbic.201402322. Epub 2014 Oct 30.

Rank ordering plate data facilitates data visualization and normalization in high-throughput screening

Authors: Mangat CS, Bharat A, Gehrke SS, Brown ED.
Reference: J Biomol Screen. 2014 Oct;19(9):1314-20. doi: 10.1177/1087057114534298. Epub 2014 May 14.

Discovery of a small molecule that inhibits bacterial ribosome biogenesis

Authors: Stokes JM, Davis JH, Mangat CS, Williamson JR, Brown ED.
Reference: Elife. 2014 Sep 18;3:e03574. doi: 10.7554/eLife.03574.

Discovery of novel cell wall-active compounds using PywaC, a sensitive reporter of cell wall stress, in the model gram-positive bacterium Bacillus subtilis

Authors: Czarny TL, Perri AL, French S, Brown ED
Reference: Antimicrob Agents Chemother. 2014 Jun;58(6):3261-9. doi: 10.1128/AAC.02352-14

Phenotypic investigations of the depletion of EngA in Escherichia coli are consistent with a role in ribosome biogenesis

Authors: Bharat A, Brown ED.
Reference: FEMS Microbiol Lett. 2014 Apr;353(1):26-32.

Designing analogs of ticlopidine, a wall teichoic acid inhibitor, to avoid formation of its oxidative metabolites.

Authors: Farha MA, Koteva K, Gale RT, Sewell EW, Wright GD, Brown ED.
Reference: Bioorg Med Chem Lett. 2014 Feb 1;24(3):905-10. doi: 10.1016/j.bmcl.2013.12.069. Epub 2013 Dec 23.

Metal-induced isomerization yields an intracellular chelator that disrupts bacterial iron homeostasis

Authors: Falconer SB, Wang W, Gehrke SS, Cuneo JD, Britten JF, Wright GD, Brown ED.
Reference: Chem Biol. 2014 Jan 16;21(1):136-45. doi: 10.1016/j.chembiol.2013.11.007. Epub 2013 Dec 19.

2013

Featured
Exploring intermolecular interactions of a substrate binding protein using a riboswitch-based sensor

Authors: Fowler CC, Sugiman-Marangos S, Junop MS, Brown ED, Li Y.
Reference: Chem Biol. 2013 Dec 19;20(12):1502-12. doi: 10.1016/j.chembiol.2013.10.014. Epub 2013 Nov 27.

Antibiotic resistance-the need for global solutions

Authors: Laxminarayan R, Duse A, Wattal C, Zaidi AK, Wertheim HF, Sumpradit N, Vlieghe E, Hara GL, Gould IM, Goossens H, Greko C, So AD, Bigdeli M, Tomson G, Woodhouse W, Ombaka E, Peralta AQ, Qamar FN, Mir F, Kariuki S, Bhutta ZA, Coates A, Bergstrom R, Wright GD, Brown ED, Cars O.
Reference: Lancet Infect Dis. 2013 Dec;13(12):1057-98. doi: 10.1016/S1473-3099(13)70318-9.

Metabolic suppression identifies new antibacterial inhibitors under nutrient limitation

Authors: Zlitni S, Ferruccio LF, Brown ED.
Reference: Nat Chem Biol. 2013 Dec;9(12):796-804. doi: 10.1038/nchembio.1361. Epub 2013 Oct 13.

Taking aim at wall teichoic acid synthesis: new biology and new leads for antibiotics

Authors: Sewell EW, Brown ED.
Reference: J Antibiot (Tokyo). 2014 Jan;67(1):43-51. doi: 10.1038/ja.2013.100. Epub 2013 Oct 30. Review.

Collapsing the proton motive force to identify synergistic combinations against Staphylococcus aureus

Authors: Farha MA, Verschoor CP, Bowdish D, Brown ED.
Reference: Chem Biol. 2013 Sep 19;20(9):1168-78. doi: 10.1016/j.chembiol.2013.07.006. Epub 2013 Aug 22.

A high-throughput screen of the GTPase activity of Escherichia coli EngA to find an inhibitor of bacterial ribosome biogenesis

Authors: Bharat A, Blanchard JE, Brown ED.
Reference: J Biomol Screen. 2013 Aug;18(7):830-6. doi: 10.1177/1087057113486001. Epub 2013 Apr 19.

Degradation of MAC13243 and studies of the interaction of resulting thiourea compounds with the lipoprotein targeting chaperone LolA

Authors: Barker CA, Allison SE, Zlitni S, Nguyen ND, Das R, Melacini G, Capretta AA, Brown ED.
Reference: Bioorg Med Chem Lett. 2013 Apr 15;23(8):2426-31. doi: 10.1016/j.bmcl.2013.02.005. Epub 2013 Feb 13. Erratum in: Bioorg Med Chem Lett. 2013 Aug 1;23(15):4469-70.

Is the GAIN Act a turning point in new antibiotic discovery?

Authors: Brown ED.
Reference: Can J Microbiol. 2013 Mar;59(3):153-6. doi: 10.1139/cjm-2013-0089. Epub 2013 Feb 6.

Discovery of antibiotic adjuvants

Authors: Farha MA, Brown ED. 
Reference: Nat Biotechnol. 2013 Feb;31(2):120-2. doi: 10.1038/nbt.2500

Inhibition of WTA synthesis blocks the cooperative action of PBPs and sensitizes MRSA to β-lactams

Authors: Farha MA, Leung A, Sewell EW, D'Elia MA, Allison SE, Ejim L, Pereira PM, Pinho MG, Wright GD, Brown ED.
Reference: ACS Chem Biol. 2013 Jan 18;8(1):226-33. doi: 10.1021/cb300413m. Epub 2012 Oct 19.

2012

Featured
Identification of drugs including a dopamine receptor antagonist that selectively target cancer stem cells

Authors: Sachlos E, Risueño RM, Laronde S, Shapovalova Z, Lee JH, Russell J, Malig M, McNicol JD, Fiebig-Comyn A, Graham M, Levadoux-Martin M, Lee JB, Giacomelli AO, Hassell JA, Fischer-Russell D, Trus MR, Foley R, Leber B, Xenocostas A, Brown ED, Collins TJ, Bhatia M.
Reference: Cell. 2012 Jun 8;149(6):1284-97. doi: 10.1016/j.cell.2012.03.049. Epub 2012 May 24.

2011

Featured
Cryo-electron microscopy structure of the 30S subunit in complex with the YjeQ biogenesis factor.

Authors: Jomaa A, Stewart G, Mears JA, Kireeva I, Brown ED, Ortega J.
Reference: RNA. 2011 Nov;17(11):2026-38. doi: 10.1261/rna.2922311. Epub 2011 Sep 29.

Studies of the genetics, function, and kinetic mechanism of TagE, the wall teichoic acid glycosyltransferase in Bacillus subtilis 168

Authors: Allison SE, D'Elia MA, Arar S, Monteiro MA, Brown ED.
Reference: J Biol Chem. 2011 Jul 8;286(27):23708-16. doi: 10.1074/jbc.M111.241265. Epub 2011 May 10.

Antibiotics as probes of biological complexity

Authors: Falconer SB, Czarny TL, Brown ED.
Reference: Nat Chem Biol. 2011 Jul;7(7):415-23. doi: 10.1038/nchembio.590. Review. No abstract available.

Combinations of antibiotics and nonantibiotic drugs enhance antimicrobial efficacy

Authors: Ejim L, Farha MA, Falconer SB, Wildenhain J, Coombes BK, Tyers M, Brown ED, Wright GD.
Reference: Nat Chem Biol. 2011 Jun;7(6):348-50. doi: 10.1038/nchembio.559. Epub 2011 Apr 24.

Understanding ribosome assembly: the structure of in vivo assembled immature 30S subunits revealed by cryo-electron microscopy

Authors: Jomaa A, Stewart G, Martín-Benito J, Zielke R, Campbell TL, Maddock JR, Brown ED, Ortega J.
Reference: RNA. 2011 Apr;17(4):697-709. doi: 10.1261/rna.2509811. Epub 2011 Feb 8.

2010

Featured
Thermodynamic and NMR analysis of inhibitor binding to dihydrofolate reductase

Authors: Batruch I, Javasky E, Brown ED, Organ MG, Johnson PE.
Reference: Bioorg Med Chem. 2010 Dec 15;18(24):8485-92. doi: 10.1016/j.bmc.2010.10.048. Epub 2010 Oct 26.

Chemical probes of Escherichia coli uncovered through chemical-chemical interaction profiling with compounds of known biological activity

Authors: Farha MA, Brown ED.
Reference: Chem Biol. 2010 Aug 27;17(8):852-62. doi: 10.1016/j.chembiol.2010.06.008.

Using a riboswitch sensor to examine coenzyme B(12) metabolism and transport in E. coli

Authors: Fowler CC, Brown ED, Li Y.
Reference: Chem Biol. 2010 Jul 30;17(7):756-65. doi: 10.1016/j.chembiol.2010.05.025.

An allosteric inhibitor of substrate recognition by the SCF(Cdc4) ubiquitin ligase.

Authors: Orlicky S, Tang X, Neduva V, Elowe N, Brown ED, Sicheri F, Tyers M.
Reference: Nat Biotechnol. 2010 Jul;28(7):733-7. doi: 10.1038/nbt.1646. Epub 2010 Jun 27.

Chemical genomic approaches to study model microbes

Authors: Barker CA, Farha MA, Brown ED.
Reference: Chem Biol. 2010 Jun 25;17(6):624-32. doi: 10.1016/j.chembiol.2010.05.010. Review.

A high-throughput pharmacoviral approach identifies novel oncolytic virus sensitizers

Authors: Diallo JS, Le Boeuf F, Lai F, Cox J, Vaha-Koskela M, Abdelbary H, MacTavish H, Waite K, Falls T, Wang J, Brown R, Blanchard JE, Brown ED, Kirn DH, Hiscott J, Atkins H, Lichty BD, Bell JC.
Reference: Mol Ther. 2010 Jun;18(6):1123-9. doi: 10.1038/mt.2010.67. Epub 2010 Apr 13.

Structure of the bacterial teichoic acid polymerase TagF provides insights into membrane association and catalysis

Authors: Lovering AL, Lin LY, Sewell EW, Spreter T, Brown ED, Strynadka NC.
Reference: Nat Struct Mol Biol. 2010 May;17(5):582-9. doi: 10.1038/nsmb.1819. Epub 2010 Apr 18.

2009

Featured
Chemical genomics in Escherichia coli identifies an inhibitor of bacterial lipoprotein targeting

Authors: Pathania R, Zlitni S, Barker C, Das R, Gerritsma DA, Lebert J, Awuah E, Melacini G, Capretta FA, Brown ED.
Reference: Nat Chem Biol. 2009 Nov;5(11):849-56. doi: 10.1038/nchembio.221. Epub 2009 Sep 27.

New screens and targets in antibacterial drug discovery

Authors: Falconer SB, Brown ED.
Reference: Curr Opin Microbiol. 2009 Oct;12(5):497-504. doi: 10.1016/j.mib.2009.07.001. Epub 2009 Aug 3. Review

Are essential genes really essential?

Authors: D'Elia MA, Pereira MP, Brown ED.
Reference: Trends Microbiol. 2009 Oct;17(10):433-8. doi: 10.1016/j.tim.2009.08.005. Epub 2009 Sep 16.

The wall teichoic acid polymerase TagF is non-processive in vitro and amenable to study using steady state kinetic analysis

Authors: Sewell EW, Pereira MP, Brown ED.
Reference: J Biol Chem. 2009 Aug 7;284(32):21132-8. doi: 10.1074/jbc.M109.010215. Epub 2009 Jun 11.

High-throughput screening identifies novel inhibitors of the acetyltransferase activity of Escherichia coli GlmU

Authors: Pereira MP, Blanchard JE, Murphy C, Roderick SL, Brown ED.
Reference: Antimicrob Agents Chemother. 2009 Jun;53(6):2306-11. doi: 10.1128/AAC.01572-08. Epub 2009 Apr 6.

The N-acetylmannosamine transferase catalyzes the first committed step of teichoic acid assembly in Bacillus subtilis and Staphylococcus aureus

Authors: D'Elia MA, Henderson JA, Beveridge TJ, Heinrichs DE, Brown ED.
Reference: J Bacteriol. 2009 Jun;191(12):4030-4. doi: 10.1128/JB.00611-08. Epub 2009 Apr 17.

Probing teichoic acid genetics with bioactive molecules reveals new interactions among diverse processes in bacterial cell wall biogenesis

Authors: D'Elia MA, Millar KE, Bhavsar AP, Tomljenovic AM, Hutter B, Schaab C, Moreno-Hagelsieb G, Brown ED.
Reference: Chem Biol. 2009 May 29;16(5):548-56. doi: 10.1016/j.chembiol.2009.04.009.

High-throughput screening of model bacteria

Authors: Zlitni S, Blanchard JE, Brown ED.
Reference: Methods Mol Biol. 2009;486:13-27. doi: 10.1007/978-1-60327-545-3_2.

Drug discovery: Not as fab as we thought

Authors: Zlitni S, Brown ED.
Reference: Nature. 2009 Mar 5;458(7234):39-40. doi: 10.1038/458039a

The ATPase activity of an 'essential' Bacillus subtilis enzyme, YdiB, is required for its cellular function and is modulated by oligomerization

Authors: Karst JC, Foucher AE, Campbell TL, Di Guilmi AM, Stroebel D, Mangat CS, Brown ED, Jault JM
Reference:  Microbiology. 2009 Mar;155(Pt 3):944-56. doi: 10.1099/mic.0.021543-0.

Identification of a toxic peptide through bidirectional expression of small RNAs

Authors: Mok WW, Navani NK, Barker C, Sawchyn BL, Gu J, Pathania R, Zhu RD, Brown ED, Li Y.
Reference: Chembiochem. 2009 Jan 26;10(2):238-41. doi: 10.1002/cbic.200800591.

2008

Featured
Ribosome biogenesis; the KsgA protein throws a methyl-mediated switch in ribosome assembly.

Authors: Mangat CS, Brown ED.
Reference: Mol Microbiol. 2008 Dec;70(5):1051-3.

Identification of pharmacological chaperones for Gaucher disease and characterization of their effects on beta-glucocerebrosidase by hydrogen/deuterium exchange mass spectrometry.

Authors: Tropak MB, Kornhaber GJ, Rigat BA, Maegawa GH, Buttner JD, Blanchard JE, Murphy C, Tuske SJ, Coales SJ, Hamuro Y, Brown ED, Mahuran DJ.
Reference: Chembiochem. 2008 Nov 3;9(16):2650-62. doi: 10.1002/cbic.200800304.

Use of CDP-glycerol as an alternate acceptor for the teichoic acid polymerase reveals that membrane association regulates polymer length.

Authors: Schertzer JW, Brown ED.
Reference: J Bacteriol. 2008 Nov;190(21):6940-7. doi: 10.1128/JB.00851-08. Epub 2008 Aug 22.

A FACS-based approach to engineering artificial riboswitches.

Authors: Fowler CC, Brown ED, Li Y.
Reference: Chembiochem. 2008 Aug 11;9(12):1906-11. doi: 10.1002/cbic.200700713

Duplication of teichoic acid biosynthetic genes in Staphylococcus aureus leads to functionally redundant poly(ribitol phosphate) polymerases.

Authors: Pereira MP, D'Elia MA, Troczynska J, Brown ED.
Reference: J Bacteriol. 2008 Aug;190(16):5642-9. doi: 10.1128/JB.00526-08. Epub 2008 Jun 13.

The wall teichoic acid polymerase TagF efficiently synthesizes poly(glycerol phosphate) on the TagB product lipid III.

Authors: Pereira MP, Schertzer JW, D'Elia MA, Koteva KP, Hughes DW, Wright GD, Brown ED.
Reference: Chembiochem. 2008 Jun 16;9(9):1385-90. doi: 10.1002/cbic.200800026

Genetic interaction screens with ordered overexpression and deletion clone sets implicate the Escherichia coli GTPase YjeQ in late ribosome biogenesis.

Authors: Campbell TL, Brown ED.
Reference: J Bacteriol. 2008 Apr;190(7):2537-45. doi: 10.1128/JB.01744-07. Epub 2008 Jan 25.

Small and lethal: searching for new antibacterial compounds with novel modes of action.

Authors: Pathania R, Brown ED.
Reference: Biochem Cell Biol. 2008 Apr;86(2):111-5. doi: 10.1139/O08-011. Review.

Canadian Chemical Biology Network: biochemistry back to the future.

Authors: Brown ED, Thomas DY.
Reference: Biochem Cell Biol. 2008 Apr;86(2):ix, xi-xii. doi: 10.1139/o08-903. 

2007

Featured
Isolation of DNA aptamers for CDP-ribitol synthase, and characterization of their inhibitory and structural properties.

Authors: Allali-Hassani A, Pereira MP, Navani NK, Brown ED, Li Y.
Reference: Chembiochem. 2007 Nov 23;8(17):2052-7.

The Amino terminus of Bacillus subtilis TagB possesses separable localization and functional properties.

Authors:  Bhavsar AP, D'Elia MA, Sahakian TD, Brown ED.
Reference: J Bacteriol. 2007 Oct;189(19):6816-23. Epub 2007 Jul 27.

Inhibitors of bacterial cystathionine beta-lyase: leads for new antimicrobial agents and probes of enzyme structure and function.

Authors: Ejim LJ, Blanchard JE, Koteva KP, Sumerfield R, Elowe NH, Chechetto JD, Brown ED, Junop MS, Wright GD.
Reference: J Med Chem. 2007 Feb 22;50(4):755-64.

Isolation of the rstA gene as a multicopy suppressor of YjeE, an essential ATPase of unknown function in Escherichia coli.

Authors: Campbell TL, Ederer CS, Allali-Hassani A, Brown ED.
Reference: J Bacteriol. 2007 Apr;189(8):3318-21. Epub 2007 Feb 9.

High-throughput screening for human lysosomal beta-N-Acetyl hexosaminidase inhibitors acting as pharmacological chaperones.

Authors: Tropak MB, Blanchard JE, Withers SG, Brown ED, Mahuran D.
Reference: Chem Biol. 2007 Feb;14(2):153-64.

2006

Featured
A 2.13 A structure of E. coli dihydrofolate reductase bound to a novel competitive inhibitor reveals a new binding surface involving the M20 loop region.

Authors: Summerfield RL, Daigle DM, Mayer S, Mallik D, Hughes DW, Jackson SG, Sulek M, Organ MG, Brown ED, Junop MS.
Reference: J Med Chem. 2006 Nov 30;49(24):6977-86.

Wall teichoic acid polymers are dispensable for cell viability in Bacillus subtilis.

Authors: D'Elia MA, Millar KE, Beveridge TJ, Brown ED.
Reference: J Bacteriol. 2006 Dec;188(23):8313-6. Epub 2006 Sep 29.

The worm turns for antimicrobial discovery.

Authors: Bhavsar AP, Brown ED.
Reference: Nat Biotechnol. 2006 Sep;24(9):1098-100. 

Cooperative and critical roles for both G domains in the GTPase activity and cellular function of ribosome-associated Escherichia coli EngA.

Authors: Bharat A, Jiang M, Sullivan SM, Maddock JR, Brown ED.
Reference: J Bacteriol. 2006 Nov;188(22):7992-6. Epub 2006 Sep 8.

Non-ribosomal factors in ribosome subunit assembly are emerging targets for new antibacterial drugs.

Authors: Comartin DJ, Brown ED.
Reference: Curr Opin Pharmacol. 2006 Oct;6(5):453-8. Epub 2006 Aug 4. Review.

The yjeQ gene is required for virulence of Staphylococcus aureus.

Authors: Campbell TL, Henderson J, Heinrichs DE,
Reference: Infect Immun. 2006 Aug;74(8):4918-21.

Small-molecule screening made simple for a difficult target with a signaling nucleic acid aptamer that reports on deaminase activity.

Authors: Elowe NH, Nutiu R, Allali-Hassani A, Cechetto JD, Hughes DW, Li Y, Brown ED.
Reference: Angew Chem Int Ed Engl. 2006 Aug 25;45(34):5648-52. No abstract available.

Cell wall assembly in Bacillus subtilis: how spirals and spaces challenge paradigms.

Authors: Bhavsar AP, Brown ED.
Reference: Mol Microbiol. 2006 Jun;60(5):1077-90.

Microbiology: antibiotic stops 'ping-pong' match.

Authors: Brown ED.
Reference: Nature. 2006 May 18;441(7091):293-4.

Crystal structure of CTP:glycerol-3-phosphate cytidylyltransferase from Staphylococcus aureus: examination of structural basis for kinetic mechanism.

Authors: Fong DH, Yim VC, D'Elia MA, Brown ED, Berghuis AM.
Reference: Biochim Biophys Acta. 2006 Jan;1764(1):63-9. Epub 2005 Nov 10.

2005

Featured
Conserved P-loop GTPases of unknown function in bacteria: an emerging and vital ensemble in bacterial physiology.

Authors:  Brown ED.
Reference:  Biochem Cell Biol. 2005 Dec;83(6):738-46. Review.

Experimental screening of dihydrofolate reductase yields a "test set" of 50,000 small molecules for a computational data-mining and docking competition.

Authors: Elowe NH, Blanchard JE, Cechetto JD, Brown ED.
Reference: J Biomol Screen. 2005 Oct;10(7):653-7. Epub 2005 Sep 16.

The TagB protein in Bacillus subtilis 168 is an intracellular peripheral membrane protein that can incorporate glycerol phosphate onto a membrane-bound acceptor in vitro.

Authors: Bhavsar AP, Truant R, Brown ED.
Reference: J Biol Chem. 2005 Nov 4;280(44):36691-700. Epub 2005 Sep 2.

Two conserved histidine residues are critical to the function of the TagF-like family of enzymes.

Authors: Schertzer JW, Bhavsar AP, Brown ED.
Reference: J Biol Chem. 2005 Nov 4;280(44):36683-90. Epub 2005 Sep 1.

Characterization of the Bacillus subtilis GTPase YloQ and its role in ribosome function.

Authors: Campbell TL, Daigle DM, Brown ED.
Reference: Biochem J. 2005 Aug 1;389(Pt 3):843-52.

New targets and screening approaches in antimicrobial drug discovery.

Authors: Brown ED, Wright GD.
Reference: Chem Rev. 2005 Feb;105(2):759-74. 

2004

Featured
Probing the active site of YjeE: a vital Escherichia coli protein of unknown function.

Authors: Allali-Hassani A, Campbell TL, Ho A, Schertzer JW, Brown ED.
References: Biochem J. 2004 Dec 15;384(Pt 3):577-84.

Teichoic acid is an essential polymer in Bacillus subtilis that is functionally distinct from teichuronic acid.

Authors: Bhavsar AP, Erdman LK, Schertzer JW, Brown ED.
Reference: J Bacteriol. 2004 Dec;186(23):7865-73.

Multicopy suppressors for novel antibacterial compounds reveal targets and drug efflux susceptibility.

Authors: Li X, Zolli-Juran M, Cechetto JD, Daigle DM, Wright GD, Brown ED.
Reference: Chem Biol. 2004 Oct;11(10):1423-30.

High-throughput screening identifies inhibitors of the SARS coronavirus main proteinase.

Authors: Blanchard JE, Elowe NH, Huitema C, Fortin PD, Cechetto JD, Eltis LD, Brown ED.
Reference: Chem Biol. 2004 Oct;11(10):1445-53

Drugs against superbugs: private lessons from bacteriophages.

Authors: Brown ED.
Reference: Trends Biotechnol. 2004 Sep;22(9):434-6.

An expedient and facile one-step synthesis of a biguanide library by microwave irradiation coupled with simple product filtration. Inhibitors of dihydrofolate reductase.

Authors: Mayer S, Daigle DM, Brown ED, Khatri J, Organ MG.
Reference: J Comb Chem. 2004 Sep-Oct;6(5):776-82.

Bifunctional catalysis by CDP-ribitol synthase: convergent recruitment of reductase and cytidylyltransferase activities in Haemophilus influenzae and Staphylococcus aureus.

Authors: Pereira MP, Brown ED.
Reference: Biochemistry. 2004 Sep 21;43(37):11802-12

Studies of the interaction of Escherichia coli YjeQ with the ribosome in vitro.

Authors: Daigle DM, Brown ED.
Reference: J Bacteriol. 2004 Mar;186(5):1381-7.

2003

Featured
High throughput screening identifies novel inhibitors of Escherichia coli dihydrofolate reductase that are competitive with dihydrofolate.

Authors: Zolli-Juran M, Cechetto JD, Hartlen R, Daigle DM, Brown ED.
Reference: Bioorg Med Chem Lett. 2003 Aug 4;13(15):2493-6.

Screening in academe: a perspective on implementation of university-based small molecule screening.

Authors: Brown ED.
Reference: J Biomol Screen. 2003 Aug;8(4):377-9.

Purified, recombinant TagF protein from Bacillus subtilis 168 catalyzes the polymerization of glycerol phosphate onto a membrane acceptor in vitro.

Authors: Schertzer JW, Brown ED.
Reference: J Biol Chem. 2003 May 16;278(20):18002-7. Epub 2003 Mar 12

CTP:glycerol 3-phosphate cytidylyltransferase (TarD) from Staphylococcus aureus catalyzes the cytidylyl transfer via an ordered Bi-Bi reaction mechanism with micromolar K(m) values.

Authors: Badurina DS, Zolli-Juran M, Brown ED.
Reference: Biochim Biophys Acta. 2003 Mar 21;1646(1-2):196-206.

2002

Featured
Characterization of the depletion of 2-C-methyl-D-erythritol-2,4-cyclodiphosphate synthase in Escherichia coli and Bacillus subtilis.

Authors: Campbell TL, Brown ED.
Reference: J Bacteriol. 2002 Oct;184(20):5609-18.

YjeQ, an essential, conserved, uncharacterized protein from Escherichia coli, is an unusual GTPase with circularly permuted G-motifs and marked burst kinetics.

Authors: Daigle DM, Rossi L, Berghuis AM, Aravind L, Koonin EV, Brown ED.
Reference:  Biochemistry. 2002 Sep 17;41(37):11109-17.